The ParaSight-F rapid dipstick antigen capture assay for monitoring parasite clearance after drug treatment of Plasmodium falciparum malaria

Trans R Soc Trop Med Hyg. Jul-Aug 1997;91(4):403-5. doi: 10.1016/s0035-9203(97)90257-1.

Abstract

Three methods for the detection of Plasmodium falciparum infection in peripheral blood were compared during antimalarial treatment and follow-up in 32 Burundian patients: dipstick antigen capture assay, standard (TBF) and prolonged thick blood film examination (PTBF) (3 x 5 min and 3 x 20 min examination respectively). Parasitaemia was determined daily by comparison with total white blood cell counts (determined by Coulter counter) until no parasite was detected on 2 consecutive days by PTBF. Cumulatively, 231 observations were made with each assay: 64 were negative and 167 positive by PTBF (59 had parasite counts < or = 100/microL). Compared to PTBF, the sensitivities of TBF and the dipstick assay were 1.0 for parasite counts > 100/microL and 0.458 and 0.966 respectively for counts < or = 100/microL. Overall, the dipstick assay was significantly more sensitive (0.988 vs. 0.808; P < 0.001) but less specific (P = 0.013) than TBF. The dipstick assay is of potential use for monitoring response to drug treatment and for detecting low parasitaemias.

MeSH terms

  • Adolescent
  • Adult
  • Animals
  • Antigens, Protozoan / analysis*
  • Antimalarials / therapeutic use
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Infant
  • Leukocyte Count
  • Malaria, Falciparum / blood
  • Malaria, Falciparum / diagnosis
  • Malaria, Falciparum / drug therapy*
  • Male
  • Plasmodium falciparum / immunology
  • Proteins / analysis
  • Protozoan Proteins / blood
  • Reagent Strips*
  • Sensitivity and Specificity
  • Treatment Outcome

Substances

  • Antigens, Protozoan
  • Antimalarials
  • Proteins
  • Protozoan Proteins
  • Reagent Strips
  • histidine-rich proteins