The effect of treatment with budesonide or PGE2 in vitro on allergen-induced increases in canine bone marrow progenitors

Am J Respir Cell Mol Biol. 1997 Nov;17(5):634-41. doi: 10.1165/ajrcmb.17.5.2746.


Increased bone marrow granulocyte-macrophage colony forming units (GM-CFU) in dogs developing allergen-induced airway hyperresponsiveness can be accounted for by a factor(s) present in serum following the allergen challenge. The present study evaluated whether in vitro treatment of bone marrow with budesonide or prostaglandin (PG)E2, prevents allergen-induced bone marrow stimulation. Eight dogs were studied after allergen and diluent inhalation challenges. Budesonide (10[-7] M) or PGE2 (10[-6] M) was added to bone marrow aspirated 24 h after challenge. Budesonide or PGE2 was also added to bone marrow aspirated before challenge, to which serum taken 24 h after challenge was subsequently added. Non-adherent mononuclear bone marrow cells were incubated in the presence of the serum and granulocyte/macrophage colony stimulating factor (GM-CSF), granulocyte stimulating factor (G-CSF), or stem cell factor (SCF), and the number of GM-CFU counted. Allergen-induced increases in the number of GM-CFU in bone marrow aspirated 24 h after allergen (P < 0.001) were not attenuated by budesonide or PGE2 treatment (P > 0.05). However, GM-CFU increases in bone marrow aspirated before challenge and incubated with post-allergen challenge serum (P < 0.001) were blocked by either budesonide or PGE2 (P < 0.001). These findings demonstrate that budesonide and PGE2 can act directly on the bone marrow, preventing allergen-induced increases in inflammatory cell progenitor production. This suggests that the bone marrow must be considered as a possible site of action for drugs which attenuate allergen-induced asthmatic responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / administration & dosage*
  • Animals
  • Bone Marrow Cells / drug effects
  • Bone Marrow Cells / pathology*
  • Bronchodilator Agents / pharmacology*
  • Budesonide / pharmacology*
  • Cell Count / drug effects
  • Cells, Cultured
  • Dinoprostone / pharmacology*
  • Dogs
  • Hematopoietic Stem Cells / drug effects
  • Hematopoietic Stem Cells / pathology*
  • Oxytocics / pharmacology*


  • Allergens
  • Bronchodilator Agents
  • Oxytocics
  • Budesonide
  • Dinoprostone