CD1d-restricted and TCR-mediated activation of valpha14 NKT cells by glycosylceramides

Science. 1997 Nov 28;278(5343):1626-9. doi: 10.1126/science.278.5343.1626.

Abstract

Natural killer T (NKT) lymphocytes express an invariant T cell antigen receptor (TCR) encoded by the Valpha14 and Jalpha281 gene segments. A glycosylceramide-containing alpha-anomeric sugar with a longer fatty acyl chain (C26) and sphingosine base (C18) was identified as a ligand for this TCR. Glycosylceramide-mediated proliferative responses of Valpha14 NKT cells were abrogated by treatment with chloroquine-concanamycin A or by monoclonal antibodies against CD1d/Vbeta8, CD40/CD40L, or B7/CTLA-4/CD28, but not by interference with the function of a transporter-associated protein. Thus, this lymphocyte shares distinct recognition systems with either T or NK cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD1 / immunology*
  • Carbohydrate Conformation
  • Cells, Cultured
  • Ceramides / chemistry
  • Ceramides / metabolism
  • Ceramides / pharmacology*
  • Cerebrosides / chemistry
  • Cerebrosides / metabolism
  • Cerebrosides / pharmacology*
  • Coculture Techniques
  • Galactosylceramides / chemistry
  • Galactosylceramides / metabolism
  • Galactosylceramides / pharmacology
  • Glucosylceramides / chemistry
  • Glucosylceramides / metabolism
  • Glucosylceramides / pharmacology
  • Killer Cells, Natural / immunology*
  • Ligands
  • Lymphocyte Activation*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Receptors, Antigen, T-Cell, alpha-beta / immunology*
  • Structure-Activity Relationship
  • T-Lymphocyte Subsets / immunology*

Substances

  • Antigens, CD1
  • Ceramides
  • Cerebrosides
  • Galactosylceramides
  • Glucosylceramides
  • Ligands
  • Receptors, Antigen, T-Cell, alpha-beta