Tissue specific control regions of the N-methyl-D-aspartate receptor subunit NR2C promoter

Biol Chem. 1997 Aug;378(8):929-34.


In the mouse brain, the N-methyl-D-aspartate receptor subunit NR2C (epsilon-3) is mainly detected in the cerebellar granule cells starting from the second week of postnatal life. In order to improve our understanding of molecular mechanisms of this neuron-specific, spatial and temporal gene expression, different promoter fragments were used to control indicator genes in nondifferentiated rat pheochromocytoma (PC12) cells, in human embryonal kidney (HEK293) cells and in transgenic mice. A 400 bp NR2C promoter region upstream of the transcriptional start site was identified as a basal promoter that was negatively regulated possibly by a neuron restrictive silencer element (NRSE) that is localized 664 base pairs downstream from the transcriptional start sites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Chemistry / genetics*
  • Gene Expression Regulation, Developmental / physiology
  • Humans
  • Luciferases / metabolism
  • Mice
  • Mice, Transgenic
  • PC12 Cells
  • Promoter Regions, Genetic / genetics*
  • Rats
  • Receptors, N-Methyl-D-Aspartate / genetics*
  • Transcription, Genetic
  • Tumor Cells, Cultured


  • Receptors, N-Methyl-D-Aspartate
  • Luciferases