GAL4-responsive UAS-tau as a Tool for Studying the Anatomy and Development of the Drosophila Central Nervous System

Cell Tissue Res. 1997 Oct;290(1):1-10. doi: 10.1007/s004410050901.

Abstract

To improve the quality of cytoplasmic labelling of GAL4-expressing cells in Drosophila enhancer-trap and transgenic strains, a new GAL4-responsive reporter UAS-tau, which features a bovine tau cDNA under control of a yeast upstream activation sequence (UAS), was tested. Tau, a microtubule-associated protein, is distributed actively and evenly into all cellular processes. Monoclonal anti-bovine Tau antibody reveals the axonal structure of the labelled cells with detail similar to that of Golgi impregnation. We demonstrate that the UAS-tau system is especially useful for studying processes of differentiation and reorganisation of identified neurones during postembryonic development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Antibodies, Monoclonal
  • Cattle
  • Central Nervous System / anatomy & histology
  • Central Nervous System / cytology
  • Central Nervous System / growth & development
  • DNA-Binding Proteins / genetics
  • Drosophila / anatomy & histology
  • Drosophila / cytology
  • Drosophila / growth & development*
  • Enhancer Elements, Genetic / physiology
  • Fungal Proteins / genetics*
  • Gene Expression Regulation, Developmental / physiology
  • Genes, Reporter
  • Genetic Testing / methods
  • Metamorphosis, Biological / physiology
  • Neurons / physiology
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors*
  • tau Proteins / genetics*
  • tau Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • DNA-Binding Proteins
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • tau Proteins