Opioid regulation of AP-1 DNA-binding activity in NG108-15 cells under conditions of opioid-receptor adaptation

Brain Res Mol Brain Res. 1997 Aug;48(1):156-8. doi: 10.1016/s0169-328x(97)00128-9.

Abstract

Opioid-receptor adaptation may lead to changes in transcriptional regulation by sequence-specific DNA-binding proteins. Gel-shift assays of nuclear extracts from NG108-15 cells revealed that an increase of AP-1 DNA-binding activity ensues under conditions previously established to induce down- or up-regulation of delta-opioid receptors.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Nucleus / drug effects
  • Cell Nucleus / metabolism
  • Consensus Sequence
  • Down-Regulation / drug effects
  • Enkephalin, Leucine-2-Alanine / pharmacology*
  • Glioma
  • Hybrid Cells
  • Mice
  • Naltrexone / pharmacology*
  • Neuroblastoma
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / metabolism*
  • Rats
  • Receptors, Opioid, delta / biosynthesis*
  • Transcription Factor AP-1 / metabolism*
  • Tumor Cells, Cultured
  • Up-Regulation / drug effects

Substances

  • Oligodeoxyribonucleotides
  • Receptors, Opioid, delta
  • Transcription Factor AP-1
  • Naltrexone
  • Enkephalin, Leucine-2-Alanine