Impaired CD19 expression and signaling, enhanced antibody response to type II T independent antigen and reduction of B-1 cells in CD81-deficient mice

Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):10844-9. doi: 10.1073/pnas.94.20.10844.

Abstract

The tetraspanin CD81 is ubiquitously expressed and associated with CD19 on B lymphocytes and with CD4 and CD8 on T lymphocytes. Analysis of mice with disrupted CD81 gene reveals normal T cells but a distinct abnormality in B cells consisting of decreased expression of CD19 and severe reduction in peritoneal B-1 cells. CD81-deficient B cells responded normally to surface IgM crosslinking, but had severely impaired calcium influx following CD19 engagement. CD81-deficient mice had increased serum IgM and IgA and an exaggerated antibody response to the type II T independent antigen TNP-Ficoll. These results suggest that CD81 is important for CD19 signaling and B cell function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibody Formation
  • Antigens, CD / genetics*
  • Antigens, CD19 / genetics
  • Antigens, CD19 / metabolism*
  • Antigens, T-Independent / immunology*
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • Immunoglobulin A / blood
  • Immunoglobulin M / blood
  • Lymphocyte Depletion
  • Membrane Proteins*
  • Mice
  • Signal Transduction*
  • Tetraspanin 28

Substances

  • Antigens, CD
  • Antigens, CD19
  • Antigens, T-Independent
  • Cd81 protein, mouse
  • Immunoglobulin A
  • Immunoglobulin M
  • Membrane Proteins
  • Tetraspanin 28