Using a eukaryotic GST fusion vector for proteins difficult to express in E. coli

Biotechniques. 1997 Nov;23(5):794-6, 798, 800. doi: 10.2144/97235bm06.

Authors

R Y Tsai¹, R R Reed

Affiliation

¹ Johns Hopkins University School of Medicine, Baltimore, MD, USA.

PMID: 9383538
DOI: 10.2144/97235bm06

No abstract available

MeSH terms

Amino Acid Sequence
Base Sequence
Binding Sites
Cell Line
Cyclic AMP-Dependent Protein Kinases / metabolism
Cytomegalovirus / genetics
Escherichia coli / genetics*
Gene Expression*
Genetic Vectors*
Glutathione Transferase / chemistry
Glutathione Transferase / genetics*
Helix-Loop-Helix Motifs
Humans
Molecular Sequence Data
Phosphorylation
Promoter Regions, Genetic
Recombinant Fusion Proteins*
Simian virus 40 / genetics
Zinc Fingers

Substances

Recombinant Fusion Proteins
Glutathione Transferase
Cyclic AMP-Dependent Protein Kinases