Comparative studies on tetrachloroethene reductive dechlorination mediated by Desulfitobacterium sp. strain PCE-S

Arch Microbiol. 1997 Dec;168(6):513-9. doi: 10.1007/s002030050529.


Tetrachloroethene reductive dechlorination was studied with cell extracts of a newly isolated, tetrachloroethene-utilizing bacterium, Desulfitobacterium sp. strain PCE-S. Tetrachloroethene dehalogenase mediated the reductive dechlorination of tetrachloroethene and trichloroethene to cis-1,2-dichloroethene with artificial electron donors such as methyl viologen. The chlorinated aromatic compounds tested so far were not reduced. A low-potential electron donor (E0' < -0.4 V) was required for tetrachloroethene reduction. The enzyme in its reduced state was inactivated by propyl iodide and reactivated by light, indicating the involvement of a corrinoid in reductive tetrachloroethene dechlorination.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria, Anaerobic / drug effects
  • Bacteria, Anaerobic / enzymology*
  • Bacteria, Anaerobic / metabolism*
  • Biodegradation, Environmental
  • Chelating Agents / pharmacology
  • Coloring Agents / pharmacology
  • Energy Metabolism / drug effects
  • Enzyme Activation / drug effects
  • Enzyme Inhibitors / pharmacology
  • Hydrolases / antagonists & inhibitors
  • Hydrolases / metabolism
  • Indoles / pharmacology
  • Oxidation-Reduction
  • Tetrachloroethylene / metabolism*


  • Chelating Agents
  • Coloring Agents
  • Enzyme Inhibitors
  • Indoles
  • n-propyl Astra blue
  • Hydrolases
  • 2,3,5,6-tetrachloro-4-hydroquinone reductive dehalogenase
  • Tetrachloroethylene