Cloning and characterisation of the adenosyl phosphosulphate kinase gene from Aspergillus nidulans

Curr Genet. 1997 Dec;32(6):408-12. doi: 10.1007/s002940050295.


The sD gene of Aspergillus nidulans has been cloned by heterologous screening of rationally selected cosmids. Co-transformation of the sD50 mutant JMP1 confirmed the presence of a functional gene. Sequence analysis determined this gene to be 680 bp in length, containing a 59-bp intron and encoding a protein of 206 amino acids. A protein-sequence comparison revealed a similarity to the C-terminal region of ATP sulphurylase, the sC gene product. Further sequence comparison revealed differences in a consensus sequence ATP-binding motif, indicating non-functionality of the APS kinase-like domain of ATP sulphurylase, and confirms sD as the gene encoding APS kinase in A. nidulans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Aspergillus nidulans / enzymology
  • Aspergillus nidulans / genetics*
  • Base Sequence
  • Cloning, Molecular
  • Gene Expression Regulation, Fungal / physiology
  • Genes, Fungal / genetics*
  • Methionine / pharmacology
  • Molecular Sequence Data
  • Phosphotransferases (Alcohol Group Acceptor) / genetics*
  • RNA, Fungal / analysis
  • RNA, Messenger / analysis
  • Restriction Mapping
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sulfate Adenylyltransferase / genetics


  • RNA, Fungal
  • RNA, Messenger
  • Methionine
  • Phosphotransferases (Alcohol Group Acceptor)
  • adenylylsulfate kinase
  • Sulfate Adenylyltransferase

Associated data

  • GENBANK/Y08866