Exogenous sphingosine 1-phosphate induces neurite retraction possibly through a cell surface receptor in PC12 cells

Biochem Biophys Res Commun. 1997 Nov 17;240(2):329-34. doi: 10.1006/bbrc.1997.7666.


Exogenous sphingosine 1-phosphate (S1P), like lysophosphatidic acid (LPA), induced neurite retraction or cell rounding in differentiated PC12 cells. The lysosphingolipid-induced shape change was detected at as low as 1 nM; however, a significant accumulation of intracellular S1P was not detected until 1 microM S1P was applied. Moreover, although exogenous sphingosine caused a significant increase in intracellular S1P by sphingosine kinase-catalyzed phosphorylation, the effect on the shape change was marginal. Exposure of the cells to the immobilized S1P in which the lipid was covalently linked to a glass carrier also resulted in the shape change. These results suggest that the exogenous S1P-induced shape change does not require uptake of the lipid into the cells but possibly requires interaction with a cell surface receptor in the neuronal cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Size / drug effects
  • Lysophospholipids / pharmacology
  • Neurites / physiology*
  • PC12 Cells
  • Phosphorylation
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism
  • Rats
  • Receptors, Cell Surface / drug effects
  • Receptors, Cell Surface / physiology*
  • Sphingosine / analogs & derivatives*
  • Sphingosine / metabolism
  • Sphingosine / pharmacology
  • Tetradecanoylphorbol Acetate / pharmacology


  • Lysophospholipids
  • Receptors, Cell Surface
  • sphingosine 1-phosphate
  • Phosphotransferases (Alcohol Group Acceptor)
  • sphingosine kinase
  • Sphingosine
  • Tetradecanoylphorbol Acetate