Family of Ebf/Olf-1-related genes potentially involved in neuronal differentiation and regional specification in the central nervous system

Dev Dyn. 1997 Nov;210(3):191-205. doi: 10.1002/(SICI)1097-0177(199711)210:3<191::AID-AJA1>3.0.CO;2-B.

Abstract

Two novel mouse genes, Ebf2 and Ebf3, have been identified which show high similarity to the rodent Ebf/Olf-1 and the Drosophila collier genes. The strong conservation of the protein regions corresponding to the DNA binding and dimerisation domains previously defined in Ebf strongly suggests that Ebf2 and Ebf3 also constitute DNA sequence-specific transcription factors. Determination of the chromosomal locations of the two genes indicated that the different members of this novel mouse multigene family are not clustered. A detailed analysis of the expression of each of the three Ebf genes in the developing central nervous system revealed partially overlapping patterns with two salient features: 1) In the region extending from the midbrain to the spinal cord, the expression of the three genes correlated with neuronal maturation, with a general activation in early post-mitotic cells, followed by specific patterns of extinction also consistent with the neurogenic gradient. 2) In the forebrain area, although the patterns of expression of the Ebf genes also reflected neuronal maturation, they appeared in addition to be region specific. These data suggest that Ebf genes may be involved in the control of neuronal differentiation in the CNS and in enforcing regional diversity in populations of post-mitotic forebrain neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Cell Differentiation / genetics
  • Central Nervous System / embryology*
  • Chromosome Mapping
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / physiology*
  • Drosophila Proteins*
  • Helix-Loop-Helix Motifs
  • Mice
  • Molecular Sequence Data
  • Neurons / cytology*
  • Phenotype
  • Prosencephalon / embryology
  • Rhombencephalon / embryology
  • Spinal Cord / embryology
  • Trans-Activators / chemistry
  • Trans-Activators / physiology*
  • Transcription Factors / chemistry
  • Transcription Factors / physiology*
  • Transcription, Genetic

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Ebf1 protein, mouse
  • Ebf2 protein, mouse
  • Ebf3 protein, mouse
  • Trans-Activators
  • Transcription Factors
  • kn protein, Drosophila

Associated data

  • GENBANK/AF041138
  • GENBANK/U82441
  • GENBANK/U82442