New monoclonal antibodies to oestrogen and progesterone receptors effective for paraffin section immunohistochemistry

J Pathol. 1997 Oct;183(2):228-32. doi: 10.1002/(SICI)1096-9896(199710)183:2<228::AID-PATH895>3.0.CO;2-X.

Abstract

Assessment of oestrogen and progesterone receptors (ER and PgR) in breast cancer is widely used for the prediction of response to endocrine therapy and as a prognostic marker. Cytosolic assays have been replaced in many centres by immunochemical techniques, which have many advantages including applicability to small samples, simplicity, and cost-effectiveness. This study describes the generation and characterisation of two novel murine monoclonal antibodies recognizing ER and PgR, designated NCL-ER-6F11 and NCL-PGR respectively, which are effective in heat-treated formalin-fixed, paraffin-embedded tissue. The antibodies have been characterized by Western blotting and by immunohistochemistry on normal and pathological breast and other tissues. NCL-ER-6F11 has been shown to compare favourably with a currently available ER antibody. These antibodies may prove of value in the assessment of hormone receptor status in human breast cancer.

MeSH terms

  • Antibodies, Monoclonal / biosynthesis*
  • Biomarkers, Tumor / immunology*
  • Biomarkers, Tumor / metabolism
  • Blotting, Western
  • Breast Neoplasms / metabolism*
  • Female
  • Humans
  • Immunohistochemistry
  • Paraffin Embedding
  • Receptors, Estrogen / immunology*
  • Receptors, Estrogen / metabolism
  • Receptors, Progesterone / immunology*
  • Receptors, Progesterone / metabolism
  • Tumor Cells, Cultured

Substances

  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • Receptors, Estrogen
  • Receptors, Progesterone