Neuropilin-semaphorin III/D-mediated chemorepulsive signals play a crucial role in peripheral nerve projection in mice

Neuron. 1997 Nov;19(5):995-1005. doi: 10.1016/s0896-6273(00)80392-x.


Neuropilin is a neuronal cell surface protein and has been shown to function as a receptor for a secreted protein, semaphorin III/D, that can induce neuronal growth cone collapse and repulsion of neurites in vitro. The roles of neuropilin in vivo, however, are unknown. Here, we report that neuropilin-deficient mutant mice produced by targeted disruption of the neuropilin gene show severe abnormalities in the trajectory of efferent fibers of the PNS. We also describe that neuropilin-deprived dorsal root ganglion neurons are perfectly protected from growth cone collapse elicited by semaphorin III/D. Our results indicate that neuropilin-semaphorin III/D-mediated chemorepulsive signals play a major role in guidance of PNS efferents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / physiology
  • Cranial Nerves / physiology
  • Extremities / innervation
  • Ganglia, Spinal / physiology
  • Glycoproteins / physiology*
  • Mice
  • Mice, Inbred ICR
  • Molecular Sequence Data
  • Mutation
  • Nerve Fibers / physiology
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / physiology*
  • Nervous System Physiological Phenomena
  • Neuropilin-1
  • Olfactory Pathways / physiology
  • Peripheral Nerves / physiology*
  • Semaphorin-3A
  • Signal Transduction / physiology*
  • Spinal Cord / physiology
  • Synaptic Transmission / physiology*


  • Glycoproteins
  • Nerve Tissue Proteins
  • Semaphorin-3A
  • Neuropilin-1

Associated data

  • GENBANK/D85028