GLUT-1 expression in the cerebra of patients with Alzheimer's disease

Neurobiol Aging. Sep-Oct 1997;18(5):469-74. doi: 10.1016/s0197-4580(97)00111-5.

Abstract

To investigate the molecular basis of reduced GLUT-1 concentration of the blood-brain barrier in patients with Alzheimer's disease (AD), the GLUT-1 mass, mRNA content, and structure were studied in eight patients with AD and seven age-matched controls. The results indicate that the 55-kDa GLUT-1 is significantly reduced in AD without a significant change in GLUT-1 mRNA concentrations. Because in some animal models changes in GLUT-1 expression is associated with changes in GLUT-1 mRNA structure, the length of the poly(A) tail of the GLUT-1 mRNA was estimated with a reverse transcription-polymerase chain reaction technique. The length of poly(A) tail of GLUT-1 mRNA in AD subjects was not significantly different from the controls. It is concluded that the AD-related change in GLUT-1 expression is not the result of altered poly(A) length of GLUT-1 mRNA.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / metabolism*
  • Blotting, Northern
  • Blotting, Western
  • Brain Chemistry / physiology*
  • Capillaries / metabolism
  • Female
  • Glucose Transporter Type 1
  • Humans
  • Male
  • Monosaccharide Transport Proteins / biosynthesis*
  • Poly A / metabolism
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis

Substances

  • Glucose Transporter Type 1
  • Monosaccharide Transport Proteins
  • RNA, Messenger
  • SLC2A1 protein, human
  • Poly A