New biomarker evidence of oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus

FEBS Lett. 1997 Nov 3;417(1):150-2. doi: 10.1016/s0014-5793(97)01273-8.

Abstract

Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) has been reported to serve as a sensitive biomarker of oxidative DNA damage and also of oxidative stress. We have investigated oxidative DNA damage in patients with non-insulin-dependent diabetes mellitus (NIDDM) by urinary 8-OHdG assessments. We determined the total urinary excretion of 8-OHdG from 24 h urine samples of 81 NIDDM patients 9 years after the initial diagnosis and of 100 non-diabetic control subjects matched for age and gender. The total 24 h urinary excretion of 8-OHdG was markedly higher in NIDDM patients than in control subjects (68.2 +/- 39.4 microg vs. 49.6 +/- 37.7 microg, P = 0.001). High glycosylated hemoglobin was associated with a high level of urinary 8-OHdG. The increased excretion of urinary 8-OHdG is seen as indicating an increased systemic level of oxidative DNA damage in NIDDM patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Aged
  • Biomarkers / urine
  • Blood Glucose / metabolism
  • DNA Damage*
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / metabolism
  • Deoxyguanosine / urine
  • Diabetes Mellitus, Type 2 / metabolism
  • Diabetes Mellitus, Type 2 / urine*
  • Female
  • Glycated Hemoglobin A / metabolism
  • Humans
  • Male
  • Middle Aged
  • Oxidative Stress*
  • Sex Factors
  • Smoking

Substances

  • Biomarkers
  • Blood Glucose
  • Glycated Hemoglobin A
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine