Anti-interleukin-10 antibodies in patients with chronic active Epstein-Barr virus infection

J Infect Dis. 1997 Dec;176(6):1454-61. doi: 10.1086/514141.


The Epstein-Barr virus (EBV) genome encodes a protein in its BamHI C restriction fragment rightward open-reading frame-1 (designated BCRF1 or viral interleukin-10 [vIL-10]) that shares protein homology and biologic properties with human IL-10. Several EBV disorders are characterized by prolonged active EBV infection. Because continued EBV replication could allow for increased vIL-10, ELISA and immunoprecipitation were used to determine whether vIL-10 expression during chronic active EBV infection resulted in vIL-10 and IL-10 antibodies. IL-10 antibodies were assayed in patients diagnosed with chronic and acute infectious mononucleosis (CIM, AIM), nasopharyngeal carcinoma (NPC), and EBV-associated lymphoproliferative disease (LPD), as well as from healthy organ transplant patients and EBV-negative or EBV-positive persons. Whether anti-IL-10 antibodies could inhibit IL-10 biologic activity was determined. vIL-10 antibodies were found in CIM, NPC, and LPD patients and antibodies reactive to IL-10 were found in CIM patients. One CIM patient had IL-10 antibodies that neutralized IL-10 bioactivity in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood
  • Antibodies, Viral / immunology*
  • CHO Cells
  • Cell Line
  • Chronic Disease
  • Cricetinae
  • Enzyme-Linked Immunosorbent Assay
  • Herpesviridae Infections / immunology*
  • Herpesvirus 4, Human / genetics
  • Herpesvirus 4, Human / immunology*
  • Humans
  • Infectious Mononucleosis / immunology*
  • Interleukin-10 / analysis
  • Interleukin-10 / genetics
  • Interleukin-10 / immunology*
  • Lymphoproliferative Disorders / immunology
  • Lymphoproliferative Disorders / virology
  • Nasopharyngeal Neoplasms / immunology
  • Nasopharyngeal Neoplasms / virology
  • Neutralization Tests
  • Organ Transplantation
  • Precipitin Tests
  • Recombinant Proteins / immunology
  • Tumor Necrosis Factor-alpha / analysis
  • Viral Proteins / genetics
  • Viral Proteins / immunology*
  • Viral Proteins / metabolism


  • Antibodies, Viral
  • BCRF1 protein, Human herpesvirus 4
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Viral Proteins
  • Interleukin-10