A novel nucleic acid-binding protein that interacts with human rad51 recombinase

Nucleic Acids Res. 1997 Dec 15;25(24):4946-53. doi: 10.1093/nar/25.24.4946.


Using the yeast two-hybrid system, we isolated a cDNA encoding a novel human protein, named Pir51, that strongly interacts with human Rad51 recombinase. Analysis in vitro confirmed the interaction between Rad51 and Pir51. Pir51 mRNA is expressed in a number of human organs, most notably in testis, thymus, colon and small intestine. The Pir51 gene locus was mapped to chromosome 12p13.1-13. 2 by fluorescence in situ hybridization. The Pir51 protein was expressed in Escherichia coli and purified to near homogeneity. Biochemical analysis shows that the Pir51 protein binds both single- and double-stranded DNA, and is capable of aggregating DNA. The protein also binds RNA. The Pir51 protein may represent a new member of the multiprotein complexes postulated to carry out homologous recombination and DNA repair in mammalian cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosomes, Human, Pair 12 / genetics
  • DNA / metabolism
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / isolation & purification
  • DNA-Binding Proteins / metabolism*
  • Genes*
  • HeLa Cells
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Organ Specificity
  • Protein Binding
  • RNA / metabolism
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • RNA-Binding Proteins
  • Rad51 Recombinase
  • Recombination, Genetic
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins


  • DNA, Complementary
  • DNA-Binding Proteins
  • RAD51AP1 protein, human
  • RNA, Messenger
  • RNA-Binding Proteins
  • Saccharomyces cerevisiae Proteins
  • RNA
  • DNA
  • RAD51 protein, S cerevisiae
  • RAD51 protein, human
  • Rad51 Recombinase

Associated data

  • GENBANK/AF006259