Tumor Site-Selective Localization of an Adoptively Transferred T Cell Line Expressing a Macrophage Lectin

J Leukoc Biol. 1997 Dec;62(6):761-70. doi: 10.1002/jlb.62.6.761.

Abstract

CTLL-2 cells were transfected with an expression vector that contained cDNA of a mouse macrophage galactose/N-acetylgalactosamine-specific calcium-type lectin (MMGL) and a stable transfectant (CTL-ML) was established. These cells and mock transfectant cells (CTL-CEP) were labeled with a long-term fluorescent cell tracer, DiI. The labeled cells were intravenously injected into mice that contained established lung metastases produced by OV2944-HM-1 ovarian tumor cells. Analyses with fluorescence microscopy of a series of frozen lung sections from the recipient mice revealed that CTL-ML preferentially accumulated in the lung metastatic nodules, whereas CTL-CEP did not. Time course studies showed that the preferential accumulation was evident 3 days after adoptive transfer. We also found that OV2944-HM-1 cells bound peanut agglutinin and Vicia villosa agglutinin B4, whose sugar specificity overlaps with the specificity of MMGL. These results suggested that MMGL molecules expressed on CTLL-2 cells contributed to their selective trafficking or retention in tumor foci possibly through recognition of tumor-associated cell surface carbohydrate antigens. These results also suggested that MMGL could be used for the selective targeting of effector cells in adoptive immunotherapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer*
  • Animals
  • Asialoglycoproteins
  • Carrier Proteins / immunology*
  • Cell Division
  • Cell Movement / immunology*
  • Female
  • Lectins / immunology*
  • Lectins, C-Type*
  • Lung Neoplasms / immunology
  • Lung Neoplasms / secondary
  • Lung Neoplasms / therapy
  • Membrane Proteins*
  • Mice
  • Neoplasms, Experimental / immunology*
  • Neoplasms, Experimental / pathology*
  • Neoplasms, Experimental / therapy
  • Ovarian Neoplasms / immunology*
  • Ovarian Neoplasms / pathology*
  • Ovarian Neoplasms / therapy
  • T-Lymphocytes / immunology
  • T-Lymphocytes / pathology*

Substances

  • Asialoglycoproteins
  • Carrier Proteins
  • Clec10a protein, mouse
  • Lectins
  • Lectins, C-Type
  • Membrane Proteins