Effect of cytochalasin B and cycloheximide on the activation rate, chromosome constituent and in vitro development of porcine oocytes following parthenogenetic stimulation

Reprod Fertil Dev. 1997;9(4):441-6. doi: 10.1071/r96078.

Abstract

Activation rate, chromosome constituent and developmental pattern of porcine oocytes was examined in the presence and absence of cytochalasin B and cycloheximide following parthenogenetic stimulation. Treatment with cycloheximide after ethanol or Ca2+ ionophore treatment increased the incidence of activation. The percentage of oocytes with two or more female pronuclei was higher (P < 0.05) in oocytes treated with cytochalasin B than in control or cycloheximide-treated oocytes. Treatment with both electrical stimulation and cytochalasin B increased the incidence of diploid chromosome spreads, and accelerated development to the morula and blastocyst stage compared with the control and cycloheximide-treated groups, suggesting a role of ploidy in the development of parthenote.

MeSH terms

  • Animals
  • Cells, Cultured
  • Chromosomes / drug effects*
  • Cycloheximide / pharmacology*
  • Cytochalasin B / pharmacology*
  • Electric Stimulation
  • Female
  • Oocytes / drug effects*
  • Parthenogenesis / drug effects*
  • Stimulation, Chemical
  • Swine

Substances

  • Cytochalasin B
  • Cycloheximide