Contents of resveratrol, piceid, and their isomers in commercially available wines made from grapes cultivated in Japan

Biosci Biotechnol Biochem. 1997 Nov;61(11):1800-5. doi: 10.1271/bbb.61.1800.

Abstract

The presence of resveratrol (3,5,4'-trihydroxystilbene) and its beta-glucoside, piceid (resveratrol-3-beta-D-glucopyranoside), together with their isomers in wine appears to be one of the beneficial factors conferring a protective effect against cardiovascular disease through red wine ingestion. A total of 42 red and white wines was collected in areas from Hokkaido to Kyushu in Japan. The wines were fractionated with a C18 Sep-pak cartridge, and the active principles were eluted with ethyl acetate. Crude trans- and cis-piceid were extracted from a Chinese medicine, 'Kojohkon' (Polygonum cuspidatum), and their retention times and UV absorption were confirmed by HPLC. trans- and cis-Resveratrol, and trans- and cis-piceid were analyzed in a short C18 HPLC column, and cis-resveratrol was quantified from the amount of cis-isomer converted from authentic trans-resveratrol that had been treated by UV irradiation. The content of piceid is shown as the resveratrol equivalent. The average content of total stilbene compounds was 4.37 mg/liter in red wines, while only 0.68 mg/liter in white wines. Red wines made from Pinot noir, Merlot, and Zweigeltrebe grapes all had a high resveratrol content.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Glucosides / analysis*
  • Isomerism
  • Japan
  • Reproducibility of Results
  • Resveratrol
  • Rosales
  • Stilbenes / analysis*
  • Wine / analysis*

Substances

  • Glucosides
  • Stilbenes
  • Resveratrol
  • polydatin