Human placental fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase: its isozymic form, expression and characterization

Biosci Biotechnol Biochem. 1997 Nov;61(11):1949-52. doi: 10.1271/bbb.61.1949.

Abstract

The nucleotide sequence of 1981 bp cDNA containing the entire coding region of a human placental fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase was determined. The sequence encodes 469 amino acids and, based on homology to the rat testis enzyme, appears to be the testis-type isozyme expressed in placenta. The enzyme was expressed in Escherichia coli BL21 (DE3) by using a T7 RNA polymerase-based expression system and purified to homogeneity. The expressed enzyme was bifunctional with specific activities of 75 and 80 mU/mg of kinase and phosphatase, respectively. Kinetic parameters of the expressed enzyme are similar to those of the rat testis enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Complementary / analysis
  • DNA, Complementary / genetics
  • Female
  • Humans
  • Isoenzymes / chemistry
  • Isoenzymes / genetics*
  • Isoenzymes / metabolism
  • Kinetics
  • Male
  • Molecular Sequence Data
  • Multienzyme Complexes / chemistry
  • Multienzyme Complexes / genetics*
  • Multienzyme Complexes / metabolism
  • Phosphofructokinase-2
  • Phosphoric Monoester Hydrolases / chemistry
  • Phosphoric Monoester Hydrolases / genetics*
  • Phosphoric Monoester Hydrolases / metabolism
  • Phosphotransferases / chemistry
  • Phosphotransferases / genetics*
  • Phosphotransferases / metabolism
  • Placenta / enzymology
  • Polymerase Chain Reaction
  • Rats
  • Sequence Homology, Amino Acid
  • Testis / enzymology

Substances

  • DNA, Complementary
  • Isoenzymes
  • Multienzyme Complexes
  • Phosphotransferases
  • Phosphofructokinase-2
  • Phosphoric Monoester Hydrolases

Associated data

  • GENBANK/D49818