Panton-valentine leukocidin genes in a phage-like particle isolated from mitomycin C-treated Staphylococcus aureus V8 (ATCC 49775)

Biosci Biotechnol Biochem. 1997 Nov;61(11):1960-2. doi: 10.1271/bbb.61.1960.


The staphylococcal Panton-Valentine leukocidin (PVL) genes [lukS-PV-lukF-PV] existed in a hexagonal phage-like particle (phi PVL) isolated from mitomycin C-induced Staphylococcus aureus V8 (ATCC 49775). The genome packed in phi PVL was a linear double-stranded 40-kb DNA with single-stranded cohesive ends (cos). The [lukS-PV-lukS-PV], attP, and int (integrase gene) of phi PVL were all located very close to one another within a 4.0 kb-segment on the genome in the order given, and the segment is located at the center from the left and the right cos sites. In addition, the [lukS-PV-lukF-PV]-attP-int region contains 5 direct repeat sequences that show high similarity with the recombinase-binding sites of bacteriophages of S. aureus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacteriophages / genetics*
  • Base Sequence
  • Binding Sites
  • DNA, Bacterial / genetics
  • DNA, Bacterial / metabolism
  • Genes, Bacterial*
  • Leukocidins / genetics*
  • Mitomycin / pharmacology*
  • Molecular Sequence Data
  • Nucleic Acid Synthesis Inhibitors / pharmacology*
  • Repetitive Sequences, Nucleic Acid
  • Sequence Homology, Amino Acid
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / virology


  • DNA, Bacterial
  • Leukocidins
  • Nucleic Acid Synthesis Inhibitors
  • Mitomycin

Associated data

  • GENBANK/AB006796