A novel DNA virus (TTV) associated with elevated transaminase levels in posttransfusion hepatitis of unknown etiology

Biochem Biophys Res Commun. 1997 Dec 8;241(1):92-7. doi: 10.1006/bbrc.1997.7765.


By means of representational difference analysis, a viral clone (N22) of 500 nucleotides was isolated from serum of a patient (TT) with posttransfusion hepatitis of unknown etiology. The N22 clone showed a poor homology to any reported sequences. Oligonucleotide primers were deduced from the N22 sequence for detecting it by polymerase chain reaction. N22 sequence in serum banded at a sucrose density of 1.26 g/cm3, indicating its association with a viral particle which was designated TT virus (TTV). Since nucleic acids of TTV were sensitive to DNase I, it would be a DNA virus. TTV DNA was detected in sera from three of the five patients with posttransfusion non-A to G hepatitis, including the index case (TT). TTV DNA titers closely correlated with aminotransferase levels in the three patients. These results indicate that TTV would be a novel DNA virus with a possible capacity to induce posttransfusion non-A to G hepatitis.

MeSH terms

  • Aged
  • Alanine Transaminase / blood*
  • Amino Acid Sequence
  • Base Sequence
  • DNA Viruses / isolation & purification*
  • DNA, Viral / blood*
  • DNA, Viral / chemistry
  • Deoxyribonuclease I
  • Female
  • Hepatitis, Viral, Human / blood
  • Hepatitis, Viral, Human / etiology
  • Hepatitis, Viral, Human / virology*
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Transfusion Reaction


  • DNA, Viral
  • Alanine Transaminase
  • Deoxyribonuclease I

Associated data

  • GENBANK/AB017767