Longitudinal study of Aspergillus fumigatus strains isolated from cystic fibrosis patients

Eur J Clin Microbiol Infect Dis. 1997 Oct;16(10):747-50. doi: 10.1007/BF01709257.


The colonization over time of cystic fibrosis patients by Aspergillus fumigatus was investigated using a DNA fingerprinting method. Aspergillus fumigatus isolates collected sequentially for more than one year from six patients with cystic fibrosis were typed by Southern blot hybridization with a repetitive DNA sequence. Each cystic fibrosis patient harbored several strains of Aspergillus fumigatus that were isolated recurrently over time. Isolates collected from a cystic fibrosis patient with aspergilloma displayed the same genotype, suggesting that the infection was due to a single strain. Continuous isolation of the same genotype in another cystic fibrosis patient, however, was not correlated clinically with an Aspergillus infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Aspergillosis / epidemiology*
  • Aspergillosis / genetics*
  • Aspergillosis / prevention & control
  • Aspergillus fumigatus / genetics
  • Aspergillus fumigatus / isolation & purification*
  • Child
  • Child, Preschool
  • Cystic Fibrosis / complications*
  • DNA Fingerprinting
  • DNA, Fungal / analysis*
  • Female
  • Humans
  • Longitudinal Studies
  • Male
  • Molecular Epidemiology
  • Recurrence
  • Time Factors


  • DNA, Fungal