An effective method of completely removing contaminating genomic DNA from an RNA sample to be used for PCR

Mol Biotechnol. 1997 Oct;8(2):135-7. doi: 10.1007/BF02752257.

Abstract

A simple method for removing contaminating genomic DNA from an RNA preparation is presented. The method involves digestion of the RNA with RNase-free DNase I at room temperature followed by inactivation of the enzyme at 65 degrees C in presence of EDTA. This method produces an RNA sample that is negative for genomic DNA by PCR.

MeSH terms

  • Biochemistry / methods*
  • DNA / chemistry*
  • DNA, Complementary
  • Deoxyribonuclease I / chemistry
  • Edetic Acid / chemistry
  • Polymerase Chain Reaction / methods*
  • RNA / isolation & purification*

Substances

  • DNA, Complementary
  • RNA
  • DNA
  • Edetic Acid
  • Deoxyribonuclease I