Abstract
The use of the Bacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains of Pasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that the sacB gene can not be used as a positive selection system in P. multocida.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Genetic Markers*
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Hexosyltransferases / drug effects
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Hexosyltransferases / genetics*
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Hexosyltransferases / metabolism
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Mutation
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Pasteurella multocida / genetics*
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Pasteurella multocida / metabolism
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Selection, Genetic*
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Sucrose / metabolism
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Sucrose / pharmacology
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Transformation, Bacterial
Substances
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Genetic Markers
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Sucrose
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Hexosyltransferases
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levansucrase