The sacB gene cannot be used as a counter-selectable marker in Pasteurella multocida

Mol Biotechnol. 1997 Oct;8(2):189-91. doi: 10.1007/BF02752263.

Abstract

The use of the Bacillus subtilis sacB gene as a counter-selectable marker was assessed in serogroup A and B strains of Pasteurella multocida. Expression ofsacB failed to render any of the strains sensitive to sucrose, indicating that the sacB gene can not be used as a positive selection system in P. multocida.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genetic Markers*
  • Hexosyltransferases / drug effects
  • Hexosyltransferases / genetics*
  • Hexosyltransferases / metabolism
  • Mutation
  • Pasteurella multocida / genetics*
  • Pasteurella multocida / metabolism
  • Selection, Genetic*
  • Sucrose / metabolism
  • Sucrose / pharmacology
  • Transformation, Bacterial

Substances

  • Genetic Markers
  • Sucrose
  • Hexosyltransferases
  • levansucrase