Molecular cloning, genetic mapping, and developmental expression of a bovine transforming growth factor beta (TGF-beta) type I receptor

Mol Reprod Dev. 1998 Jan;49(1):1-9. doi: 10.1002/(SICI)1098-2795(199801)49:1<1::AID-MRD1>3.0.CO;2-U.


A full-length cDNA encoding the bovine transforming growth factor beta (TGF-beta) receptor type I (bT beta R-I) was isolated from a placenta cDNA library. The deduced protein sequence of 499 residues contains a single transmembrane domain, a cysteinerich extracellular domain, and an intracellular kinase domain with predicted serine/threonine specificity. The amino acid sequence is 96% and 95% identical with its human and mouse homologues, respectively. Genetic mapping assigned the TGFBR1 gene to bovine chromosome 8 at a male genetic distance of 2 centimorgan from D8S28. Assuming conservation of gene order, the linkage data define a breakpoint in mammalian chromosome evolution. Both TGF-beta receptor type I and II mRNAs were found to be expressed in bovine oocytes and preimplantation two-cell, four-cell, eight-cell, morula-, and blastocyst-stage embryos, as determined by heminested reverse transcription polymerase chain reaction (RT-PCR). The mRNA expression patterns of TGF-beta receptor types I, II, and III in a variety of bovine organ tissues were examined by Northern blot hybridization, and highest levels were detected in lung and ovary.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Chromosome Mapping*
  • Cloning, Molecular
  • DNA, Complementary
  • Embryonic Development
  • Female
  • Gene Expression Regulation, Developmental*
  • Humans
  • Male
  • Mice
  • Molecular Sequence Data
  • Pregnancy
  • Protein Serine-Threonine Kinases
  • Proteoglycans / genetics
  • Receptor, Transforming Growth Factor-beta Type II
  • Receptors, Transforming Growth Factor beta / biosynthesis
  • Receptors, Transforming Growth Factor beta / genetics*
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • Proteoglycans
  • Receptors, Transforming Growth Factor beta
  • betaglycan
  • Protein Serine-Threonine Kinases
  • Receptor, Transforming Growth Factor-beta Type II

Associated data

  • GENBANK/U97485