Mosquito larvicidal activity of transgenic Anabaena strain PCC 7120 expressing combinations of genes from Bacillus thuringiensis subsp. israelensis

Appl Environ Microbiol. 1997 Dec;63(12):4971-4. doi: 10.1128/aem.63.12.4971-4974.1997.

Abstract

Various combinations of the genes cryIVA (cry4A), cryIVD (cry11A), and p20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 by means of Escherichia coli-Anabaena shuttle vector pRL488p and were expressed under control of two tandem strong promoters, a cyanobacterial promoter (PpsbA) and an E. coli T7 promoter (PA1). Two of the clones carrying cryIVA plus cryIVD, one with p20 and one without p20, displayed toxicity against third-instar larvae of Aedes aegypti at levels greater than any level previously reported for transgenic cyanobacteria.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aedes
  • Anabaena / genetics*
  • Animals
  • Bacillus thuringiensis / genetics*
  • Bacillus thuringiensis Toxins
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / pharmacology
  • Bacterial Toxins*
  • Endotoxins / genetics*
  • Endotoxins / pharmacology
  • Escherichia coli / genetics
  • Gene Expression
  • Gene Transfer Techniques
  • Genes, Bacterial
  • Genetic Vectors
  • Hemolysin Proteins
  • Larva
  • Pest Control, Biological
  • Promoter Regions, Genetic

Substances

  • Bacillus thuringiensis Toxins
  • Bacterial Proteins
  • Bacterial Toxins
  • Endotoxins
  • Hemolysin Proteins
  • insecticidal crystal protein, Bacillus Thuringiensis