Abstract
Various combinations of the genes cryIVA (cry4A), cryIVD (cry11A), and p20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 by means of Escherichia coli-Anabaena shuttle vector pRL488p and were expressed under control of two tandem strong promoters, a cyanobacterial promoter (PpsbA) and an E. coli T7 promoter (PA1). Two of the clones carrying cryIVA plus cryIVD, one with p20 and one without p20, displayed toxicity against third-instar larvae of Aedes aegypti at levels greater than any level previously reported for transgenic cyanobacteria.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aedes
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Anabaena / genetics*
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Animals
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Bacillus thuringiensis / genetics*
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Bacillus thuringiensis Toxins
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Bacterial Proteins / genetics*
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Bacterial Proteins / pharmacology
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Bacterial Toxins*
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Endotoxins / genetics*
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Endotoxins / pharmacology
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Escherichia coli / genetics
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Gene Expression
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Gene Transfer Techniques
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Genes, Bacterial
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Genetic Vectors
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Hemolysin Proteins
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Larva
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Pest Control, Biological
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Promoter Regions, Genetic
Substances
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Bacillus thuringiensis Toxins
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Bacterial Proteins
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Bacterial Toxins
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Endotoxins
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Hemolysin Proteins
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insecticidal crystal protein, Bacillus Thuringiensis