Identification of cDNAs encoding two G protein-coupled receptors for lysosphingolipids

FEBS Lett. 1997 Nov 17;417(3):279-82. doi: 10.1016/s0014-5793(97)01301-x.


The structural similarity of lysosphingolipids to lysophosphatidic acid (LPA) prompted a sequence-based search for lysosphingolipid receptors using cDNA sequence of the Edg2 human LPA receptor. Two closely related G protein-coupled receptors, rat H218 and human Edg3, are highly similar to Edg2. When overexpressed in Jurkat cells, H218 and Edg3 activated serum response element-driven transcriptional reporter gene in response to sphingosine 1-phosphate (S1P), dihydro-S1P and sphingosylphosphorylcholine, but not to LPA. H218 and Edg3 expressed in Xenopus oocytes conferred responsiveness to S1P and dihydro-S1P in agonist-triggered 45Ca2+ efflux. Therefore, H218 and Edg3 are functional receptors for S1P and perhaps other closely related lysosphingolipids.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary
  • GTP-Binding Proteins / metabolism
  • Humans
  • Jurkat Cells
  • Luciferases / biosynthesis
  • Molecular Sequence Data
  • Oocytes / drug effects
  • Oocytes / physiology
  • Polymerase Chain Reaction
  • Rats
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / physiology
  • Receptors, G-Protein-Coupled*
  • Receptors, Lysophosphatidic Acid
  • Receptors, Lysophospholipid
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Sphingolipids / metabolism*
  • Sphingolipids / pharmacology
  • Xenopus


  • DNA Primers
  • DNA, Complementary
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • Receptors, Lysophosphatidic Acid
  • Receptors, Lysophospholipid
  • Recombinant Fusion Proteins
  • Sphingolipids
  • Luciferases
  • GTP-Binding Proteins

Associated data

  • GENBANK/AF022137
  • GENBANK/AF022138
  • GENBANK/AF022139