Expression cloning and characterization of a renal organic anion transporter from winter flounder

FEBS Lett. 1997 Nov 17;417(3):287-91. doi: 10.1016/s0014-5793(97)01304-5.


The cDNA coding for a renal p-aminohippurate (PAH) transporter from winter flounder (Pseudopleuronectes americanus), designated fROAT, was cloned by functional expression in Xenopus laevis oocytes. fROAT is approximately 2.8 kbp in length and encodes a protein of 562 amino acids, related to the rat renal organic anion transporter ROAT1/OAT1 and the organic cation transporters OCT1 and OCT2. In oocytes, fROAT mediated probenecid-sensitive PAH uptake, with a Km for PAH of about 20 microM, and inhibited by external glutarate (GA) (1 mM). The functional characteristics suggest that fROAT is the basolateral PAH/dicarboxylate exchanger of the flounder kidney.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anion Transport Proteins
  • Base Sequence
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / chemistry*
  • Carrier Proteins / pharmacology*
  • Cloning, Molecular
  • Female
  • Flounder
  • Gene Library
  • Kidney / metabolism*
  • Kinetics
  • Molecular Sequence Data
  • Oocytes / physiology
  • RNA, Messenger / isolation & purification
  • RNA, Messenger / metabolism
  • Rats
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Xenopus laevis
  • p-Aminohippuric Acid / pharmacokinetics*


  • Anion Transport Proteins
  • Carrier Proteins
  • RNA, Messenger
  • p-Aminohippuric Acid

Associated data

  • GENBANK/Z97028