Nuclear translocation of the Y-box binding protein by ultraviolet irradiation

FEBS Lett. 1997 Nov 17;417(3):390-4. doi: 10.1016/s0014-5793(97)01296-9.


The Y-box binding protein, YB-1, is a member of a DNA binding protein family with a structurally and functionally conserved cold shock domain. Using Western blotting and immunohistochemical methods, larger amounts of YB-1 were detected in the cytosol, particularly at the perinuclear region, than in the nucleus of human cancer cells. UV irradiation increased accumulation of YB-1 in the nucleus at 20 min and thereafter. This translocation of YB-1 into the nucleus by UV irradiation was blocked by the protein kinase inhibitor H-7, but not HA-1004. Both green fluorescent protein (GFP)-YB-1 and GFP-YB-1C with the C-terminus (248-317) of YB-1 were located mainly in the cytosol, but GFP-YB-1deltaC with a deletion at the C-terminus of YB-1 was located in the nucleus. YB-1 is translocated into the nucleus by UV irradiation, possibly through a protein kinase C-mediated signal transduction pathway, and the C-terminal region of YB-1 might be important for cytoplasmic retention of YB-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / pharmacology
  • Animals
  • CCAAT-Enhancer-Binding Proteins*
  • COS Cells
  • Cell Nucleus / metabolism*
  • Cell Nucleus / radiation effects
  • DNA-Binding Proteins / metabolism*
  • DNA-Binding Proteins / radiation effects
  • Enzyme Inhibitors / pharmacology
  • Fluorescent Antibody Technique, Indirect
  • Green Fluorescent Proteins
  • Humans
  • Isoquinolines / pharmacology
  • KB Cells / radiation effects*
  • Luminescent Proteins / metabolism
  • NFI Transcription Factors
  • Nuclear Proteins
  • Protein Kinase Inhibitors
  • Recombinant Fusion Proteins / metabolism
  • Sulfonamides*
  • Transcription Factors / metabolism
  • Transfection
  • Tumor Cells, Cultured
  • Ultraviolet Rays*
  • Urinary Bladder Neoplasms
  • Y-Box-Binding Protein 1


  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • Enzyme Inhibitors
  • Isoquinolines
  • Luminescent Proteins
  • NFI Transcription Factors
  • Nuclear Proteins
  • Protein Kinase Inhibitors
  • Recombinant Fusion Proteins
  • Sulfonamides
  • Transcription Factors
  • Y-Box-Binding Protein 1
  • YBX1 protein, human
  • Green Fluorescent Proteins
  • 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
  • N-(2-guanidinoethyl)-5-isoquinolinesulfonamide