Allergens of Aspergillus fumigatus and Candida boidinii share IgE-binding epitopes

Am J Respir Crit Care Med. 1997 Dec;156(6):1956-62. doi: 10.1164/ajrccm.156.6.9702087.


From an Aspergillus fumigatus complementary deoxyribonucleic acid (cDNA) library displayed on phage surface, an allergen formally termed rAsp f 3 was cloned. The open-reading frame of the cloned gene for the allergen encodes a protein of 168 amino acids with a predicted molecular mass of 18.5 kD, showing 36% identity and 58% similarity to two peroxisomal membrane proteins of Candida boidinii. Recombinant Asp f 3 was expressed as a [His]6-tagged fusion protein in Escherichia coil at yields of 30 mg/L, and was purified by Ni(2+)-chelate chromatography. In an enzyme-linked immunosorbent assay (ELISA), serum IgE antibody reactivity to rAsp f 3 could be detected in 72% of 89 individuals sensitized to A. fumigatus, demonstrating that the protein represents a major allergen of the mold. IgE specific to rAsp f 3 and the two recombinant Candida proteins was further demonstrated by IgE-immunoblot analysis. IgE binding to rAsp f 3 could be inhibited in the ELISA by adding either of the recombinant Candida peroxisomal proteins to sera containing IgE directed against Asp f 3. Taken together, these observations prove that the Asperigillus allergen and the two Candida proteins share IgE-binding epitopes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / immunology*
  • Amino Acid Sequence
  • Antigen-Antibody Reactions
  • Antigens, Fungal / chemistry
  • Antigens, Fungal / genetics
  • Antigens, Fungal / immunology
  • Aspergillus fumigatus / immunology*
  • Base Sequence
  • Binding Sites, Antibody*
  • Candida / immunology*
  • Epitopes / analysis*
  • Female
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / immunology
  • Humans
  • Hypersensitivity / immunology
  • Immunoglobulin E / immunology*
  • Male
  • Middle Aged


  • Allergens
  • Antigens, Fungal
  • Epitopes
  • Fungal Proteins
  • Immunoglobulin E

Associated data

  • GENBANK/U58050