Video fluorescein imaging of the skin: description of an overviewing technique for functional evaluation of regional cutaneous blood perfusion in occlusive arterial disease of the limbs

Clin Physiol. 1997 Nov;17(6):619-33. doi: 10.1046/j.1365-2281.1997.00057.x.


In peripheral arterial occlusive disease (PAOD), and particularly in critical limb ischaemia, fluorescence recording of the skin after i.v. injection of sodium fluorescein provides an overviewing functional imaging of regional blood perfusion as revealed by a continuous, almost geographical, mapping of changes in the dynamic fluorescence pattern. Based on the previously used technique of rapid sequence flash photography introduced by Lund and Lund in 1973, a new video fluorescence imaging (VFI) technique has been developed and used in clinical routine. VFI has a broad versatility with non-touch access to areas of different size, such as the feet, hands or larger limb areas. Examples of application are the diagnosis of small artery disease, predictive prognosis of critical limb ischaemia, evaluation of distal outflow in connection with arterial reconstruction or pharmacotherapy of non-reconstructable critical ischaemia, as well as in decisions on amputation and assessment of amputation level. Thanks to its overviewing qualities with reproducible mapping of blood perfusion in standardized examination conditions, VFI would seem to have a well-justified role in angiology and above all in PAOD in both research and clinical routine work.

Publication types

  • Clinical Trial

MeSH terms

  • Adult
  • Aged
  • Arterial Occlusive Diseases / diagnosis
  • Arterial Occlusive Diseases / pathology*
  • Capillary Permeability
  • Coloring Agents
  • Diagnostic Imaging*
  • Extremities / blood supply
  • Female
  • Fluorescein
  • Foot / blood supply
  • Gangrene / pathology
  • Humans
  • Ischemia / diagnosis
  • Ischemia / pathology
  • Male
  • Middle Aged
  • Regional Blood Flow / physiology
  • Skin / blood supply*
  • Videotape Recording


  • Coloring Agents
  • Fluorescein