Regulatory DNA elements of phenobarbital-responsive cytochrome P450 CYP2B genes

J Biochem Mol Toxicol. 1998;12(1):3-9. doi: 10.1002/(sici)1099-0461(1998)12:1<3::aid-jbt2>;2-p.


This article reviews recent progress in characterizing cis-acting DNA elements of the phenobarbital-inducible CYP2B genes. Whereas proximal DNA elements such as the C/EBP binding site regulate basal transcription activity, phenobarbital-responsive enhancer activity is governed by the distal element (designated phenobarbital-responsive enhancer module, PBREM) residing about -2.3 kbp upstream from the transcription start site. Proximal elements are not required to enhance the phenobarbital-inducible transcription, since the PBREM can confer the inducibility to several heterologous promoters. Repression of the basal transcription by a negative element upstream of the -0.8 kbp region, however, may be necessary for the proper regulation of the CYP2B genes.

Publication types

  • Review

MeSH terms

  • Bacillus megaterium / genetics
  • Cytochrome P-450 Enzyme System / biosynthesis
  • Cytochrome P-450 Enzyme System / genetics*
  • Enzyme Induction
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Phenobarbital / pharmacology*
  • Regulatory Sequences, Nucleic Acid*


  • Cytochrome P-450 Enzyme System
  • Phenobarbital