Induction of antigen-specific antitumor immunity with adenovirus-transduced dendritic cells

Gene Ther. 1997 Oct;4(10):1023-8. doi: 10.1038/


Transduction of dendritic cells (DC) can result in presentation of tumor-associated antigens and induction of immunity against undefined epitopes. The present studies demonstrate adenovirus (Ad)-mediated transduction of the beta-galactosidase gene in mouse DC. Similar transductions have been obtained with the gene encoding the DF3/MUC1 tumor-associated antigen. We show that the Ad-transduced DC are functional in primary allogeneic mixed lymphocyte reactions. Mice immunized with Ad-transduced DC develop cytotoxic T lymphocytes that are specific for the beta-galactosidase or DF3/MUC1 antigens. The results also demonstrate that Ad MUC1-transduced DC induce a specific response which inhibits the growth of DF3/MUC1-positive tumors. These findings support the usefulness of Ad-transduced DC for in vivo immunization against tumor-associated antigens.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Antigens, Neoplasm / genetics
  • Antigens, Neoplasm / immunology*
  • Cell Culture Techniques
  • Cytotoxicity, Immunologic
  • Dendritic Cells / immunology*
  • Epitopes / immunology
  • Immunity, Cellular
  • Lymphocyte Culture Test, Mixed
  • Mice
  • Mice, Inbred C57BL
  • Neoplasms, Experimental / immunology*
  • T-Lymphocytes, Cytotoxic / immunology
  • beta-Galactosidase / genetics
  • beta-Galactosidase / immunology


  • Antigens, Neoplasm
  • Epitopes
  • beta-Galactosidase