Expression of c-Met correlates with grade of malignancy in human astrocytic tumours: an immunohistochemical study

Histopathology. 1997 Nov;31(5):436-43. doi: 10.1046/j.1365-2559.1997.3010889.x.


Aims: Recent studies suggest the involvement of hepatocyte growth factor/scatter factor (HGF/SF) in glioma cell invasion and tumour progression. We investigated the distribution and rate of tumour cells that express c-Met protein, which is the cell-surface receptor for HGF/SF, in astrocytic tumours. The type of cells that express c-Met in tumour tissues was also identified.

Methods and results: c-Met expression was screened immunohistochemically in a total of 43 astrocytic tumours, including 14 low-grade astrocytomas (A), 13 anaplastic astrocytomas (AA) and 16 glioblastoma multiforme (GBM), c-Met reactivity was demonstrated predominantly in the cytoplasm of tumour cells. Bizarre large tumour cells tended to stain intensely. Higher c-Met expression levels (> or = 2+, more than 25% cells were positive) were noted in 21.4% of (A) vs. 53.8% in (AA) and 87.5% in (GBM) (P < 0.001), indicating a clear relationship between c-Met protein staining and higher grade astrocytic tumours. Moreover, c-Met immunoreactivity was also shown in tumour microvasculature, reactive astrocytes, and neurones in the cortex infiltrated by glioma cells. In 85.7% of cases containing infiltrated cortex, neurones were positive vs. no neurones in non-neoplastic regions (P < 0.002).

Conclusions: This evidence suggests that c-Met expression in the brain could be associated with astrocytoma progression and also reactive process. Immunohistochemical determination of c-Met-expressing cell types helps to understand possible roles of c-Met in tumour tissues.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Astrocytes / metabolism
  • Astrocytes / pathology
  • Astrocytoma / blood supply
  • Astrocytoma / metabolism*
  • Astrocytoma / pathology
  • Brain Neoplasms / blood supply
  • Brain Neoplasms / metabolism*
  • Brain Neoplasms / pathology
  • Cerebral Cortex / metabolism
  • Cerebral Cortex / pathology
  • Child
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Microcirculation / metabolism
  • Microcirculation / pathology
  • Middle Aged
  • Neoplasm Staging
  • Neurons / metabolism
  • Neurons / pathology
  • Proto-Oncogene Proteins c-met / biosynthesis*


  • Proto-Oncogene Proteins c-met