Kinetics of recovery of the dark-adapted salamander rod photoresponse

J Gen Physiol. 1998 Jan;111(1):7-37. doi: 10.1085/jgp.111.1.7.

Abstract

The kinetics of the dark-adapted salamander rod photocurrent response to flashes producing from 10 to 10(5) photoisomerizations (Phi) were investigated in normal Ringer's solution, and in a choline solution that clamps calcium near its resting level. For saturating intensities ranging from approximately 10(2) to 10(4) Phi, the recovery phases of the responses in choline were nearly invariant in form. Responses in Ringer's were similarly invariant for saturating intensities from approximately 10(3) to 10(4) Phi. In both solutions, recoveries to flashes in these intensity ranges translated on the time axis a constant amount (tauc) per e-fold increment in flash intensity, and exhibited exponentially decaying "tail phases" with time constant tauc. The difference in recovery half-times for responses in choline and Ringer's to the same saturating flash was 5-7 s. Above approximately 10(4) Phi, recoveries in both solutions were systematically slower, and translation invariance broke down. Theoretical analysis of the translation-invariant responses established that tauc must represent the time constant of inactivation of the disc-associated cascade intermediate (R*, G*, or PDE*) having the longest lifetime, and that the cGMP hydrolysis and cGMP-channel activation reactions are such as to conserve this time constant. Theoretical analysis also demonstrated that the 5-7-s shift in recovery half-times between responses in Ringer's and in choline is largely (4-6 s) accounted for by the calcium-dependent activation of guanylyl cyclase, with the residual (1-2 s) likely caused by an effect of calcium on an intermediate with a nondominant time constant. Analytical expressions for the dim-flash response in calcium clamp and Ringer's are derived, and it is shown that the difference in the responses under the two conditions can be accounted for quantitatively by cyclase activation. Application of these expressions yields an estimate of the calcium buffering capacity of the rod at rest of approximately 20, much lower than previous estimates.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium / metabolism
  • Calcium / pharmacology
  • Cyclic GMP / metabolism
  • Dark Adaptation / physiology*
  • Enzyme Activation
  • GTP-Binding Proteins / physiology
  • Guanylate Cyclase / metabolism
  • Isomerism
  • Isotonic Solutions
  • Kinetics
  • Linear Models
  • Models, Biological*
  • Ringer's Solution
  • Rod Cell Outer Segment / enzymology*
  • Rod Opsins / chemistry
  • Rod Opsins / metabolism
  • Time Factors
  • Urodela
  • Vision, Ocular / drug effects
  • Vision, Ocular / physiology*

Substances

  • Isotonic Solutions
  • Rod Opsins
  • Ringer's Solution
  • GTP-Binding Proteins
  • Guanylate Cyclase
  • Cyclic GMP
  • Calcium