Identification of a novel gene, PSD, adjacent to NFKB2/lyt-10, which contains Sec7 and pleckstrin-homology domains

Genomics. 1997 Dec 1;46(2):251-9. doi: 10.1006/geno.1997.5022.

Abstract

We have identified a novel human gene on chromosome 10q24 located contiguously to the 3' end of the NFKB2/lyt-10 gene in a tail to tail arrangement. We describe here a cDNA of 4307 bp, isolated from an adult human brain cDNA library, which contains an open reading frame encoding a putative protein of 645 amino acids with a predicted molecular weight of 71 kDa. Database homology searches indicate that this is a novel gene coding for a putative protein containing two discrete domains with significant homology to the Sec7 and pleckstrin-homology (PH) domains, respectively. We named this gene PSD (plekstrin-Sec7 domains gene). Northern blot analysis of a panel of RNAs from normal human tissues using the PSD cDNA as probe revealed the presence of three different tissue-specific transcripts of approximately 4.3, 2.3, and 1.8 kb, the longest of which is expressed only in brain. Our data suggest that the PSD gene may code for a protein related to a recently identified protein family containing both the Sec7 and the PH domains thought to be involved in signaling transduction processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blood Proteins / genetics
  • Blotting, Northern
  • Brain / physiology
  • Chromosomes, Human, Pair 10
  • Cloning, Molecular
  • Fungal Proteins / genetics
  • Guanine Nucleotide Exchange Factors*
  • Humans
  • Molecular Sequence Data
  • NF-kappa B / genetics
  • NF-kappa B p52 Subunit
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism*
  • Phosphoproteins*
  • Protein Biosynthesis
  • Proteins / genetics*
  • Proteins / metabolism*
  • RNA Splicing
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription, Genetic

Substances

  • Blood Proteins
  • Fungal Proteins
  • Guanine Nucleotide Exchange Factors
  • NF-kappa B
  • NF-kappa B p52 Subunit
  • Nerve Tissue Proteins
  • PSD protein, human
  • Phosphoproteins
  • Proteins
  • Sec7 guanine nucleotide exchange factors
  • platelet protein P47

Associated data

  • GENBANK/X99688