Map positions of 47 Arabidopsis sequences with sequence similarity to disease resistance genes

Plant J. 1997 Nov;12(5):1197-211. doi: 10.1046/j.1365-313x.1997.12051197.x.


Map positions have been determined for 42 non-redundant Arabidopsis expressed sequence tags (ESTs) showing similarity to disease resistance genes (R-ESTs), and for three Pto-like sequences that were amplified with degenerate primers. Employing a PCR-based strategy, yeast artificial chromosome (YAC) clones containing the EST sequences were identified. Since many YACs have been mapped, the locations of the R-ESTs could be inferred from the map positions of the YACs. R-EST clones that exhibited ambiguous map positions were mapped as either cleavable amplifiable polymorphic sequence (CAPS) or restriction fragment length polymorphism (RFLP) markers using F8 (Ler x Col-0) recombinant inbred (RI) lines. In all cases but two, the R-ESTs and Pto-like sequences mapped to single, unique locations. One R-EST and one Pto-like sequence each mapped to two locations. Thus, a total of 47 loci were identified in this study. Several R-ESTs occur in clusters suggesting that they may have arisen via gene duplication events. Interestingly, several R-ESTs map to regions containing genetically defined disease resistance genes. Thus, this collection of mapped R-ESTs may expedite the isolation of disease resistance genes. As the cDNA sequencing projects have identified an estimated 63% of Arabidopsis genes, a very large number of R-ESTs (approximately 95), and by inference disease resistance genes of the leucine-rich repeat-class probably occur in the Arabidopsis genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • Base Sequence
  • Chromosome Mapping* / methods
  • Chromosomes, Artificial, Yeast
  • Crosses, Genetic
  • Genes, Plant*
  • Genetic Markers
  • Immunity, Innate
  • Molecular Sequence Data
  • Plant Diseases / genetics*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Recombination, Genetic
  • Sequence Tagged Sites


  • Genetic Markers

Associated data

  • GENBANK/U82399
  • GENBANK/U82401
  • GENBANK/U82402