RNA recognition motif 2 of yeast Pab1p is required for its functional interaction with eukaryotic translation initiation factor 4G

Mol Cell Biol. 1998 Jan;18(1):51-7. doi: 10.1128/mcb.18.1.51.

Abstract

The eukaryotic mRNA 3' poly(A) tail and its associated poly(A)-binding protein (Pab1p) are important regulators of gene expression. One role for this complex in the yeast Saccharomyces cerevisiae is in translation initiation through an interaction with a 115-amino-acid region of the translation initiation factor eIF4G. The eIF4G-interacting domain of Pab1p was mapped to its second RNA recognition motif (RRM2) in an in vitro binding assay. Moreover, RRM2 of Pab1p was required for poly(A) tail-dependent translation in yeast extracts. An analysis of a site-directed Pab1p mutation which bound to eIF4G but did not stimulate translation of uncapped, polyadenylated mRNA suggested additional Pab1p-dependent events during translation initiation. These results support the model that the association of RRM2 of yeast Pab1p with eIF4G is a prerequisite for the poly(A) tail to stimulate the translation of mRNA in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Eukaryotic Initiation Factor-4G
  • Gene Expression Regulation, Fungal*
  • Peptide Initiation Factors / genetics*
  • Peptide Initiation Factors / metabolism
  • Poly(A)-Binding Proteins
  • Protein Biosynthesis
  • RNA, Fungal / genetics*
  • RNA, Fungal / metabolism
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism

Substances

  • Eukaryotic Initiation Factor-4G
  • Peptide Initiation Factors
  • Poly(A)-Binding Proteins
  • RNA, Fungal
  • RNA-Binding Proteins