Human IAP-like protein regulates programmed cell death downstream of Bcl-xL and cytochrome c

Mol Cell Biol. 1998 Jan;18(1):608-15. doi: 10.1128/mcb.18.1.608.

Abstract

The gene encoding human IAP-like protein (hILP) is one of several mammalian genes with sequence homology to the baculovirus inhibitor-of-apoptosis protein (iap) genes. Here we show that hILP can block apoptosis induced by a variety of extracellular stimuli, including UV light, chemotoxic drugs, and activation of the tumor necrosis factor and Fas receptors. hILP also protected against cell death induced by members of the caspase family, cysteine proteases which are thought to be the principal effectors of apoptosis. hILP and Bcl-xL were compared for their ability to affect several steps in the apoptotic pathway. Redistribution of cytochrome c from mitochondria, an early event in apoptosis, was not blocked by overexpression of hILP but was inhibited by Bcl-xL. In contrast, hILP, but not Bcl-xL, inhibited apoptosis induced by microinjection of cytochrome c. These data suggest that while Bcl-xL may control mitochondrial integrity, hILP can function downstream of mitochondrial events to inhibit apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Apoptosis / genetics*
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Cell Line
  • Cytochrome c Group / genetics*
  • Cytochrome c Group / metabolism
  • Escherichia coli Proteins*
  • Gene Expression Regulation*
  • Humans
  • Proto-Oncogene Proteins c-bcl-2 / genetics*
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • bcl-X Protein

Substances

  • BCL2L1 protein, human
  • Bacterial Proteins
  • Cytochrome c Group
  • Escherichia coli Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-X Protein
  • iap protein, E coli