Structure of a novel phosphocholine-containing aminoglycoglycerolipid of Mycoplasma fermentans

Biochim Biophys Acta. 1997 Nov 8;1349(1):1-12. doi: 10.1016/s0005-2760(97)00088-x.


Mycoplasma fermentans is thought to be a pathogen of rheumatoid arthritis or a cofactor of AIDS (acquired immunodeficiency syndrome). To elucidate the possible involvement of membrane constituents in the pathogenesis of these diseases, we studied its lipid components. Several alkali labile glycophospholipids were detected and named glycoglycerophospholipids (GGPLs). Previously, we purified and determined the structure of one of them as 6'-O-phosphocholine-alpha-glucopyranosyl-(1'-3)-1,2-diacyl-sn-glycerol (GGPL-I). The present paper describes the purification and structural characterization of GGPL-III, the major GGPL of M. fermentans using 1H-, 13C- and 31P-nuclear magnetic resonance spectroscopy, and mass-spectroscopy as 1"-phosphocholine,2"-amino dihydroxypropane-3"-phospho-6'-alpha-glucopyranosyl-(1'-3)-1,2-dia cyl-glycerol.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Glycolipids / chemistry*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Mycoplasma fermentans / chemistry*
  • Phosphorylcholine / chemistry*
  • Phosphorylcholine / isolation & purification


  • Glycolipids
  • Phosphorylcholine