Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 23 (6), 1094-7

Compact, Synthetic, Vaccinia Virus Early/Late Promoter for Protein Expression

Affiliations

Compact, Synthetic, Vaccinia Virus Early/Late Promoter for Protein Expression

S Chakrabarti et al. Biotechniques.

Abstract

Vaccinia virus, a member of the poxvirus family, is widely used as a mammalian cell expression vector. Vaccinia virus replicates in the cytoplasm and has its own transcriptional system, making it necessary to use viral promoters. Here, we describe the design, construction and use of a 40-bp synthetic, vaccinia virus promoter with largely overlapping early and late regulatory elements. Convenient plasmid transfer vectors are depicted for expression of one or two genes under control of strong early/late promoters and allowing for thymidine kinase (TK) or antibiotic selection of recombinant viruses.

Similar articles

See all similar articles

Cited by 167 PubMed Central articles

See all "Cited by" articles

Publication types

LinkOut - more resources

Feedback