Enzyme inhibition in human skin homogenates by hydrocortisone, hydrocortisone acetate and hydrotisone butyrate

Arch Dermatol Res. 1976 Jun 21;255(3):265-70. doi: 10.1007/BF00561497.

Abstract

In fresh human skin homogenates, the activities of four enzymes, lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G-6-PDH), "acid" phosphatase (AcP), and "leucine aminopeptidase" (LAP) were assayed following an incubation with hydrocortisone, hydrocotisone acetate, or hydrocortisone-17-butyrate, respectively. Concentration of the three compounds measured 2.75 mMol/l. Hydrocortison butyrate inhibited LDH-G-6-PDH-, and AcP-activities. Hydrocortisone and hydrocortisone acetate exerted a significant inhibitory action only in the case of G-6-PDH-activity.--On pure G-6-PDH from yeast, the inhibition exerted by hydrocortisone butyrate was significantly stronger than the inhibition exerted by the two other steroids. Time/action diagrams revealed the fact that hydrocortisone butyrate is superior to the other two compounds from the beginning of the incubation period.--The date sustain the assumption that hydrocortisone butyrate exerts biochemical-pharmacological actions of its own and that it may not be considered just as an esterified transport form of hydrocortisone.

Publication types

  • Comparative Study

MeSH terms

  • Acetates
  • Acid Phosphatase / antagonists & inhibitors
  • Administration, Topical
  • Anti-Inflammatory Agents / pharmacology
  • Butyrates
  • Glucosephosphate Dehydrogenase / antagonists & inhibitors
  • Humans
  • Hydrocortisone / analogs & derivatives*
  • Hydrocortisone / pharmacology*
  • L-Lactate Dehydrogenase / antagonists & inhibitors
  • Leucyl Aminopeptidase / antagonists & inhibitors
  • Skin / enzymology*

Substances

  • Acetates
  • Anti-Inflammatory Agents
  • Butyrates
  • L-Lactate Dehydrogenase
  • Glucosephosphate Dehydrogenase
  • Acid Phosphatase
  • Leucyl Aminopeptidase
  • Hydrocortisone