Epstein-Barr virus infection in non-carcinomatous gastric epithelium

J Pathol. 1997 Nov;183(3):293-8. doi: 10.1002/(SICI)1096-9896(199711)183:3<293::AID-PATH937>3.0.CO;2-C.


Gastric tissue specimens from 20 patients with chronic atrophic gastritis, one of whom also had an early gastric carcinoma, were studied for evidence of Epstein-Barr virus (EBV) infection by Southern blot analysis, DNA and RNA in situ hybridization, and immunohistochemistry for the presence of the EBV-determined nuclear antigen 1 (EBNA-1) and the latent membrane protein 1 (LMP-1). EBV DNA was detected in two cases with chronic atrophic gastritis and in the case with early gastric carcinoma by Southern blot hybridization. DNA in situ hybridization showed EBV genomes in the epithelial cells of two other cases with chronic atrophic gastritis and in non-carcinomatous and carcinomatous epithelial cells of the early gastric carcinoma case. EBNA-1 was detected in all cases. LMP-1 was detected in areas of intestinal metaplasia in eight patients with chronic atrophic gastritis. EBV-encoded small RNA 1 (EBER-1) expression was limited to carcinoma cells. These results show that gastric epithelium is frequently infected with EBV and suggest that prolonged EBV persistence may contribute to the development of gastric carcinoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, Viral / analysis
  • Blotting, Southern
  • DNA, Viral / analysis
  • Epstein-Barr Virus Nuclear Antigens / analysis
  • Female
  • Gastric Mucosa / virology
  • Gastritis, Atrophic / microbiology
  • Gastritis, Atrophic / pathology
  • Gastritis, Atrophic / virology*
  • Helicobacter pylori / isolation & purification
  • Herpesviridae Infections / etiology*
  • Herpesviridae Infections / pathology
  • Herpesvirus 4, Human* / genetics
  • Herpesvirus 4, Human* / immunology
  • Humans
  • Immunoenzyme Techniques
  • In Situ Hybridization
  • Male
  • Metaplasia / virology
  • Middle Aged
  • Precancerous Conditions / virology
  • RNA, Viral / analysis
  • Stomach Neoplasms / pathology
  • Stomach Neoplasms / virology*
  • Tumor Virus Infections / etiology*
  • Tumor Virus Infections / pathology
  • Viral Matrix Proteins / analysis


  • Antigens, Viral
  • DNA, Viral
  • EBV-associated membrane antigen, Epstein-Barr virus
  • Epstein-Barr Virus Nuclear Antigens
  • RNA, Viral
  • Viral Matrix Proteins