The Non-Contact Corneal Aesthesiometer (NCCA) was used to monitor the effect of topical anesthesia on corneal sensitivity loss and recovery. In a single masked randomized study, one drop of nonpreserved 0.4% benoxinate hydrochloride was instilled in either the right, left, or both eyes of 20 subjects on separate days, and measurements were made of the central corneal sensitivity in the right eye over a 60-min period. As controls, 10 subjects were retested, without any anesthetic instillation, over the same time period. No significant differences were found between these two benoxinate treatments, nor between the contralateral treatment and controls. Results indicate that there is no contralateral effect of benoxinate anesthesia, and that full recovery does not occur until 60 min after instillation. No effect on the depth and duration of anesthesia was found for iris color.