Immunochemical evidence for increased formation of advanced glycation end products and inhibition by aminoguanidine in diabetic rat lenses

Biochem Biophys Res Commun. 1997 Dec 18;241(2):352-4. doi: 10.1006/bbrc.1997.7744.


Water-soluble and urea-soluble protein fractions from control and streptozotocin-diabetic rats were analyzed for AGEs with a CML-specific monoclonal anti-AGE antibody and a polyclonal anti-AGE antibody. AGEs, CML in particular, were significantly increased in the diabetic rats whereas aminoguandine treatment resulted in significant decrease in AGEs. The data also confirm that CML, a glycoxidation product, is a major epitope of AGE structures in lenses.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibody Specificity
  • Diabetes Mellitus, Experimental / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Glycation End Products, Advanced / antagonists & inhibitors*
  • Glycation End Products, Advanced / immunology
  • Guanidines / pharmacology*
  • Lens, Crystalline / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Streptozocin


  • Glycation End Products, Advanced
  • Guanidines
  • Streptozocin
  • pimagedine