Effect of enantiomeric forms of albuterol on stimulated secretion of granular protein from human eosinophils

Pulm Pharmacol Ther. 1997;10(2):97-104. doi: 10.1006/pupt.1997.0082.


We studied the effect of R-, S- and R,S-albuterol in inhibiting the eosinophil peroxidase (EPO) secretion caused by 10(-10) to 10(-6) M formyl-met-leu-phe + 5 micrograms/ml cytochalasin B (FMLP/CB) in non-allergic and allergic subjects. Total RAST score obtained for allergic subjects was 4.12 +/- 0.21 vs 0.36 +/- 0.17 for non-allergic subjects (P < 0.0001). Stimulated EPO secretion was comparable in allergic [2,051 +/- 567 ng/10(6) eosinophils (eos)] and non-allergic subjects [2,337 +/- 488 ng/10(6) eos (P = NS)]. At all concentrations used, both R- and R,S-enantiomers caused comparable (27-32%) inhibition of FMLP/CB stimulated secretion of EPO in allergic and non-allergic subjects. Pretreatment with S-albuterol caused no augmentation of EPO secretion in either allergic (115 +/- 34.6%) or non-allergic subjects (114 +/- 23.7%) subjects, and there was no significant difference in secretion caused by FMLP/CB alone in either experimental group. Similar results were obtained for subjects stratified according to serum IgE concentration. Our data demonstrate that both R- and R,S-albuterol are equivalently effective in inhibiting stimulated secretion of EPO in both normal and allergic subjects and that there is no paradoxical augmenting effect of S-albuterol in stimulated eosinophil secretion.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adrenergic beta-Agonists / pharmacology*
  • Albuterol / pharmacology*
  • Eosinophil Peroxidase
  • Eosinophils / drug effects*
  • Eosinophils / metabolism
  • Humans
  • Immunoglobulin E / blood
  • N-Formylmethionine Leucyl-Phenylalanine
  • Peroxidases / metabolism*
  • Rhinitis, Allergic, Seasonal / blood*
  • Stereoisomerism


  • Adrenergic beta-Agonists
  • Immunoglobulin E
  • N-Formylmethionine Leucyl-Phenylalanine
  • Eosinophil Peroxidase
  • Peroxidases
  • Albuterol