mRNA encoding the catalytic subunit of DNA-dependent protein kinase is widely expressed in Xenopus cells

Gene. 1997 Dec 12;203(2):235-40. doi: 10.1016/s0378-1119(97)00498-8.

Abstract

Here, the sequence of a Xenopus laevis cDNA encoding the 640 carboxy-terminal amino acids of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is reported. The predicted Xenopus protein segment is 65% identical to the human counterpart. Northern blot analysis indicates that Xenopus DNA-PKcs is encoded by an approx. 13000 nt transcript. DNA-PKcs mRNA is widely expressed in adult tissues as well as in oocytes and embryos. It is also shown that outside the conserved kinase domain, Xenopus DNA-PKcs bears significant similarities to hypothetical 420.8 and 433.2 kDa proteins in yeast species.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Cells, Cultured
  • Cloning, Molecular
  • DNA Primers
  • DNA-Activated Protein Kinase
  • DNA-Binding Proteins*
  • Embryo, Nonmammalian / enzymology
  • Evolution, Molecular
  • Gene Expression Regulation, Developmental
  • Humans
  • Molecular Sequence Data
  • Nuclear Proteins
  • Oocytes / enzymology
  • Oogenesis
  • Protein Serine-Threonine Kinases / analysis
  • Protein Serine-Threonine Kinases / chemistry
  • Protein Serine-Threonine Kinases / genetics*
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Xenopus laevis

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • DNA-Activated Protein Kinase
  • PRKDC protein, human
  • Protein Serine-Threonine Kinases

Associated data

  • GENBANK/AF001413